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Mol Cell Proteomics. 2015 Jan

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작성자 Tina
댓글 0건 조회 2회 작성일 25-06-21 02:31

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Be certain that all the purification buffers are at four °C and carry out the protein purification in the chilly room to avoid protein denaturation. BE AWARE: This avoids the coupling of amino acids within the further cycles with the membrane instead of the rising peptide chain. NOTICE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and in addition a number of the amino groups inside the spot areas do not kind bonds with the amino acids.

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